| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jauffret, Capucine M (6)
|
MAWAC |
35.07 |
27.05 |
-8.02 |
| 2 |
Dennis, Laken B (6)
|
MAMARA |
|
28.39 |
- |
| 3 |
Jefferson, McKinley F (6)
|
MAWAC |
31.86 |
28.92 |
-2.94 |
| 4 |
Caraballo, Natalia R (6)
|
MAWAC |
48.91 |
33.71 |
-15.20 |
| 5 |
Lyons, Ella J (5)
|
MAWAC |
|
34.60 |
- |
| 6 |
Celano, Giada B (6)
|
MAWAC |
|
38.97 |
- |
| 7 |
Lyons, Abigail C (5)
|
MAWAC |
|
46.00 |
- |
| 8 |
Romeo, Victoria A (6)
|
MAWAC |
|
46.88 |
- |
| 9 |
Schroeder, Caroline K (4)
|
MAOCCC |
|
47.04 |
- |
| 10 |
Blizzard, Mackenzie E (5)
|
MAOCCC |
|
55.92 |
- |
|
Curtis, Catie P (6)
|
MADST |
42.12 |
DQ |
- |
|
Sbriglia, Isabella (6)
|
MADST |
|
DQ |
- |
|
Barron, Isabella M (6)
|
MAOCCC |
|
DQ |
- |
|
Koch, Claudia J (6)
|
MADST |
43.73 |
NS |
- |
|
Reasons, Kayla (6)
|
MAWAP |
|
NS |
- |